Gene name |
SPCC1020.09 |
Gene ID |
44/A05 |
Gene synonyms/obsolete |
|
Gene product |
similar to guanine
nucleotide-binding protein; WD repeat protein; no apparent Sc
ortholog; non-essential |
Entry clone |
Cloned in 2004
trial |
ORF length (unspliced) |
1200 |
ORF length (spliced) |
|
Entry clone length |
1200 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1020.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAATTGTGTAAACTC |
Rev primer name |
SPCC1020.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCCATCCAAACCTGTAAA |
Amino acid length |
399 |
Molecular weight |
45.7 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |