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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC25H2.15
Gene ID 44/A02
Gene synonyms/obsolete
Gene product hypothetical protein; related to proteins involved in programmed cell death
Entry clone Cloned#
ORF length (unspliced) 1191
ORF length (spliced)
Entry clone length 1191
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC25H2.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCAAAAATGGGTTCA
Rev primer name SPBC25H2.15.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCCACTGAATTCCAACC
Amino acid length 396
Molecular weight 44.2
Isoelectric point (calc.) 8.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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