| Gene name |
SPAC5H10.12c |
| Gene ID |
43/G11 |
| Gene synonyms/obsolete |
B20341-3 |
| Gene product |
GlcNac transferase;
galactosyltransferase family 8; similar to S. pombe SPBC4C3.09
and SPBC4C3.08; similar to S. cerevisiae YOR320C;
predicted N-terminal signal sequence |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1116 |
| ORF length (spliced) |
|
| Entry clone length |
1116 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC5H10.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCGAATTTCACTTTC |
| Rev primer name |
SPAC5H10.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATGAGTTTCATACTTATGA |
| Amino acid length |
371 |
| Molecular weight |
43.6 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |