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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1687.21
Gene ID 43/F01
Gene synonyms/obsolete SPAC222.01
Gene product phosphoglycerate mutase family
Entry clone Cloned
ORF length (unspliced) 927
ORF length (spliced) 630
Entry clone length 927
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1687.21.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGGTGTTCTTGATTCG
Rev primer name SPAC1687.21.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCAATTAATTTGCCAGTC
Amino acid length 209
Molecular weight 23.6
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAQRLLPLDI
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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