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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC25B8.14
Gene ID 43/E11
Gene synonyms/obsolete mal2
Gene product involved in genome stability (required); involved in chromosome segregation (required); essential; deletion mutant results in nondisjunction of sister chromatids; involved in chromatin organization at the centromere (maintenance) (required); involved in centromeric transcriptional silencing (required); inner centomere core complex; required for correct metaphase spindle length; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 912
ORF length (spliced)
Entry clone length 912
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC25B8.14.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGACGATGAAGAAGGCAA
Rev primer name SPAC25B8.14.Rv
Rev primer SEQ AGAAAGCTGGGTATAACGAAGCATGACTTTCT
Amino acid length 303
Molecular weight 34.5
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLQINKSLTI
Localization (YFP) SPB; nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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