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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC18B11.04
Gene ID 43/B11
Gene synonyms/obsolete
Gene product related to neuronal calcium sensor 1 proteins; EF hand motifs; calcium binding protein
Entry clone Cloned
ORF length (unspliced) 776
ORF length (spliced) 573
Entry clone length 776
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC18B11.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAAAATCACAATCAAA
Rev primer name SPAC18B11.04.Rv
Rev primer SEQ AGAAAGCTGGGTAAACCAATCCATCGTATAAA
Amino acid length 190
Molecular weight 22
Isoelectric point (calc.) 4.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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