Gene name |
SPBC577.04 |
Gene ID |
42/H11 |
Gene synonyms/obsolete |
|
Gene product |
eukaryotic conserved
region; no apparent Sc ortholog; predicted coiled-coil protein
|
Entry clone |
Cloned |
ORF length (unspliced) |
686 |
ORF length (spliced) |
603 |
Entry clone length |
686 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC577.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACAGAAAATGCAATATC |
Rev primer name |
SPBC577.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATGGAGGTAATGTTATAG |
Amino acid length |
200 |
Molecular weight |
23.4 |
Isoelectric point (calc.) |
9.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |