Gene name |
SPAC1F8.02c |
Gene ID |
42/H09 |
Gene synonyms/obsolete |
|
Gene product |
exoglucanase I
precursor glycoprotein; possibly Sp specific; GPI anchored
protein (pers. comm. Birgit Eisenhaber); no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
681 |
ORF length (spliced) |
|
Entry clone length |
681 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1F8.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTCTTTAAAAATTTA |
Rev primer name |
SPAC1F8.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGTTTAAAACCAAGTAA |
Amino acid length |
226 |
Molecular weight |
25 |
Isoelectric point (calc.) |
3.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSVGLYLVL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |