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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1F8.02c
Gene ID 42/H09
Gene synonyms/obsolete
Gene product exoglucanase I precursor glycoprotein; possibly Sp specific; GPI anchored protein (pers. comm. Birgit Eisenhaber); no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 681
ORF length (spliced)
Entry clone length 681
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1F8.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTTCTTTAAAAATTTA
Rev primer name SPAC1F8.02.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAGTTTAAAACCAAGTAA
Amino acid length 226
Molecular weight 25
Isoelectric point (calc.) 3.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLSVGLYLVL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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