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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC645.03c
Gene ID 42/G08
Gene synonyms/obsolete lsa1
Gene product hypothetical protein; possible involvement iniron-sulfur protein; involved in iron-sulfur cluster biosynthesis; HesB-like domain; involved in iron metabolism; involved in sulfur metabolism
Entry clone Cloned
ORF length (unspliced) 661
ORF length (spliced) 573
Entry clone length 661
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC645.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTGAGTTGTCTTTCGAA
Rev primer name SPCC645.03.Rv
Rev primer SEQ AGAAAGCTGGGTACTTAAGAGTACTAAACGAT
Amino acid length 190
Molecular weight 21.2
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQFLRKLSL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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