| Gene name |
SPCC1183.08c |
| Gene ID |
42/F10 |
| Gene synonyms/obsolete |
rpl101; rpl1-1;
rpl10a-2 |
| Gene product |
60S ribosomal protein
L10A |
| Entry clone |
Cloned |
| ORF length (unspliced) |
651 |
| ORF length (spliced) |
|
| Entry clone length |
651 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC1183.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAAGGTCAGCGTTGC |
| Rev primer name |
SPCC1183.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTAAAGTCTGTGAGGCTTA |
| Amino acid length |
216 |
| Molecular weight |
23.9 |
| Isoelectric point (calc.) |
10.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
89 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
| Microscope used for
observation |
Leica |