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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1805.13
Gene ID 42/F08
Gene synonyms/obsolete rpl14
Gene product 60S ribosomal protein L14
Entry clone Cloned
ORF length (unspliced) 649
ORF length (spliced) 405
Entry clone length 649
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC1805.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAGGATTCAAGCGTTA
Rev primer name SPAC1805.13.Rv
Rev primer SEQ AGAAAGCTGGGTAGGCCTTGAGAGCCTTAGCG
Amino acid length 134
Molecular weight 15.2
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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