| Gene name |
SPCC330.03c |
| Gene ID |
42/E11 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical heme
binding protein; cytochrome b5 family; heme-binding domain;
similar to Sp SPAC1F12.10C (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
632 |
| ORF length (spliced) |
438 |
| Entry clone length |
632 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC330.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGTCGATTTTCAAAAA |
| Rev primer name |
SPCC330.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACACCAAGTAACCAACAAAA |
| Amino acid length |
145 |
| Molecular weight |
16.3 |
| Isoelectric point (calc.) |
9.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
32 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
periphery; cytoplasmic
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |