| Gene name |
SPAC22F3.02 |
| Gene ID |
42/E08 |
| Gene synonyms/obsolete |
atf31 |
| Gene product |
putative transcription
factorbZIP (basic leucine zipper) transcription factor family;
overexpression suppresses calcium sensitivity of Atf1
deletants; non-essential; involved in regulation of meiosis;
involved in regulation of transcription from PolII promoter;
transcription factor activity; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
630 |
| ORF length (spliced) |
|
| Entry clone length |
630 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC22F3.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACCTACGAAACAAATAC |
| Rev primer name |
SPAC22F3.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGAGGCGTAAGCGGTTTT |
| Amino acid length |
209 |
| Molecular weight |
23.9 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus; nuclear
dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |