Gene name |
SPBC660.17c |
Gene ID |
42/E01 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
615 |
ORF length (spliced) |
519 |
Entry clone length |
615 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC660.17.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAACAATTACGAAAAAG |
Rev primer name |
SPBC660.17.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATATGAGCAAAACGCATC |
Amino acid length |
172 |
Molecular weight |
20.5 |
Isoelectric point (calc.) |
5.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
4 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYLLLIVPLFL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
Zeiss |