Gene name |
SPCC126.13c |
Gene ID |
42/D07 |
Gene synonyms/obsolete |
|
Gene product |
similar to sin3
associated polypeptide; involved in transcriptional
regulation; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
605 |
ORF length (spliced) |
438 |
Entry clone length |
605 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC126.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATATTCGTGAAAGTCG |
Rev primer name |
SPCC126.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTTTTTATTCTAATTTTA |
Amino acid length |
145 |
Molecular weight |
16.8 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGVLIADQLLL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |