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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP27G11.13c
Gene ID 42/A01
Gene synonyms/obsolete
Gene product small nuclear ribonucleoprotein complex; involved in rRNA modification; involved in 35S primary transcript processing; direct rRNA pseudouridinylation
Entry clone Cloned
ORF length (unspliced) 247
ORF length (spliced) 195
Entry clone length 247
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAP27G11.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCATTTGATGTATTATTT
Rev primer name SPAP27G11.13.Rv
Rev primer SEQ AGAAAGCTGGGTAGTAAGGTTTTGCAGCTAAT
Amino acid length 64
Molecular weight 7.6
Isoelectric point (calc.) 10.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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