Gene name |
SPBC336.02 |
Gene ID |
41/H06 |
Gene synonyms/obsolete |
dim1 |
Gene product |
dimethylase;
dimethyladenosine transferase; 18S rRNA dimethylase;
essential; required for mitosis; interacts physically with
Lid1p; required for APC function; involved in mRNA
splicing |
Entry clone |
Cloned |
ORF length (unspliced) |
982 |
ORF length (spliced) |
924 |
Entry clone length |
982 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC336.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAAAGATTCGGGTTAG |
Rev primer name |
SPBC336.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCAAAATGAACGCCAACC |
Amino acid length |
307 |
Molecular weight |
34.6 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleolus>>nucleus; spindle microtubules |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleolus>nucleus) |
Microscope used for
observation |
Leica |