Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3F6.03
Gene ID 41/H04
Gene synonyms/obsolete trr1; caf4
Gene product thioredoxin reductase
Entry clone Cloned
ORF length (unspliced) 1268
ORF length (spliced) 969
Entry clone length 969
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3F6.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTCACAACAAGGTTGT
Rev primer name SPBC3F6.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATCGGTATCTTCCAATTCT
Amino acid length 322
Molecular weight 34.6
Isoelectric point (calc.) 4.9
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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