Gene name |
SPAPB2B4.02 |
Gene ID |
41/F05 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; PICOT domain |
Entry clone |
Cloned |
ORF length (unspliced) |
773 |
ORF length (spliced) |
441 |
Entry clone length |
773 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
186A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAPB2B4.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATTCGATGTTTAGATT |
Rev primer name |
SPAPB2B4.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCCTTTTTAGTAGTTTCC |
Amino acid length |
146 |
Molecular weight |
16.5 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKAIQILSL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |