Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB2B4.02
Gene ID 41/F05
Gene synonyms/obsolete
Gene product conserved hypothetical; PICOT domain
Entry clone Cloned
ORF length (unspliced) 773
ORF length (spliced) 441
Entry clone length 773
No. of intron 1
Sequence status Finished
Sequence results 186A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB2B4.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATTCGATGTTTAGATT
Rev primer name SPAPB2B4.02.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCTTTTTAGTAGTTTCC
Amino acid length 146
Molecular weight 16.5
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKAIQILSL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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