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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC776.16
Gene ID 41/F02
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned#
ORF length (unspliced) 762
ORF length (spliced)
Entry clone length 762
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC776.16.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTGGAACAAAGTGAATC
Rev primer name SPBC776.16.Rv
Rev primer SEQ AGAAAGCTGGGTACCTTCTACGACTTCCCTTA
Amino acid length 253
Molecular weight 28.7
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQTFGKLIL
Localization (YFP) SPB; nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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