Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC589.06c
Gene ID 41/E12
Gene synonyms/obsolete
Gene product involved in the regulation of inorganic phosphate transport; involved in phosphate metabolism
Entry clone Cloned
ORF length (unspliced) 751
ORF length (spliced) 609
Entry clone length 751
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC589.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAACGCTCAGGTAAGTTT
Rev primer name SPAC589.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAAGACTTTTCAGTTTCG
Amino acid length 202
Molecular weight 21.7
Isoelectric point (calc.) 5.9
Signal SEQ
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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