Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB1E7.12
Gene ID 41/E05
Gene synonyms/obsolete rps602; rps6-2; rps6
Gene product 40S ribosomal protein S6
Entry clone Cloned
ORF length (unspliced) 720
ORF length (spliced)
Entry clone length 720
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB1E7.12.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGCTGAACATTTCCTA
Rev primer name SPAPB1E7.12.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTTCATAGAAGAAGCA
Amino acid length 239
Molecular weight 27.4
Isoelectric point (calc.) 11.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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