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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPJ4664.05
Gene ID 41/C09
Gene synonyms/obsolete
Gene product conserved hypothetical; conserved protein
Entry clone Cloned
ORF length (unspliced) 492
ORF length (spliced)
Entry clone length 492
No. of intron 0
Sequence status Finished
Sequence results 437T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBPJ4664.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAGTAGGATTATTTTC
Rev primer name SPBPJ4664.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGGATCTTGGAGGTGCACCA
Amino acid length 163
Molecular weight 18.4
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain 3
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LFSDISGSLTL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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