Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3H5.10
Gene ID 41/B04
Gene synonyms/obsolete rpl3202; rpl32; rpl32-2
Gene product 60S ribosomal protein L32
Entry clone Cloned
ORF length (unspliced) 384
ORF length (spliced)
Entry clone length 384
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3H5.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTGCTGTCAATATCAT
Rev primer name SPAC3H5.10.Rv
Rev primer SEQ AGAAAGCTGGGTACTCCTGAGAGCGAACCTTA
Amino acid length 127
Molecular weight 14.4
Isoelectric point (calc.) 11.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.