| Gene name |
SPAC13C5.07 |
| Gene ID |
40/H02 |
| Gene synonyms/obsolete |
rad32 |
| Gene product |
exonuclease; involved
in DNA repair; involved in double-strand break repair
(required); involved in meiotic recombination (required);
involved in meiotic gene conversion (required); involved in
end joining; involved in telomere maintenance; involved in
telomerase-dependent telomere maintenance; involved in DNA
damage sensitivity; involved in DNA replication; Mre11 complex
(multisubunit endonuclease); phosphoprotein; essential
phosphoesterase motif |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
2156 |
| ORF length (spliced) |
1950 |
| Entry clone length |
2156 |
| No. of intron |
4 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC13C5.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCAAATGACCCCTCAGA |
| Rev primer name |
SPAC13C5.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATCATCTAAAATTTCGTCA |
| Amino acid length |
649 |
| Molecular weight |
73.6 |
| Isoelectric point (calc.) |
5.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
574 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYQALRSLRL/LQKGFTKLAL |
| Localization (YFP) |
no expression clone
|
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|