Gene name |
SPAC688.13 |
Gene ID |
40/C02 |
Gene synonyms/obsolete |
scn1 |
Gene product |
interacts physically
with Cut9p; TatD DNase family |
Entry clone |
Cloned; Mixture |
ORF length (unspliced) |
1008 |
ORF length (spliced) |
|
Entry clone length |
1008 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
Mixture from (-5) /
583A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC688.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATCTATCATTGACGC |
Rev primer name |
SPAC688.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTTACTTTCAAAAATGCT |
Amino acid length |
335 |
Molecular weight |
38.4 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |