| Gene name |
SPBC337.16 |
| Gene ID |
40/A09 |
| Gene synonyms/obsolete |
cho1 |
| Gene product |
phospholipid
methyltransferase; phosphatidyl-N-methylethanolamine
N-methyltransferase; involved in phosphatidylcholine
biosynthesis; phosphatidyl-N-methylethanolamine
N-methyltransferase activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
721 |
| ORF length (spliced) |
666 |
| Entry clone length |
721 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC337.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCTTGATTCTTTACCC |
| Rev primer name |
SPBC337.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTCGGATTTTTTAGCTTGC |
| Amino acid length |
221 |
| Molecular weight |
24.7 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
3 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIFCFGSVLVL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |