| Gene name |
SPBC3B9.07c |
| Gene ID |
40/A05 |
| Gene synonyms/obsolete |
rpa43; rpa21 |
| Gene product |
DNA-directed RNA
polymerase I (subunit); interacts physically with Rrn3p |
| Entry clone |
Cloned |
| ORF length (unspliced) |
694 |
| ORF length (spliced) |
522 |
| Entry clone length |
694 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3B9.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCGGATTTATCACTCTA |
| Rev primer name |
SPBC3B9.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAATTTGCTAGCGTCCCT |
| Amino acid length |
173 |
| Molecular weight |
19.3 |
| Isoelectric point (calc.) |
5.2 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |