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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC2F12.03c
Gene ID 38/H08
Gene synonyms/obsolete
Gene product TPR repeat protein; EST1 domain; similar to Sp SPBC2D10.13
Entry clone Cloned
ORF length (unspliced) 2676
ORF length (spliced)
Entry clone length 2676
No. of intron 0
Sequence status Finished
Sequence results 598A:G / 1132G:A / 2192T:C / 2256A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC2F12.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCGCCATAGATTCATG
Rev primer name SPBC2F12.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATTATTGCTGATGTTAACC
Amino acid length 891
Molecular weight 98.5
Isoelectric point (calc.) 8.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSDSVNSQLPL
Localization (YFP) cytosol; cytoplasmic dots; periphery at site of septum formation
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>>cytosol; cytoplasmic dots
Microscope used for observation Leica

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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