Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22F8.05
Gene ID 38/H06
Gene synonyms/obsolete
Gene product alpha,alpha-trehalose-phosphate synthase; glycosyl transferase family 20; similar to Sp SPACUNK4.16c (paralog)
Entry clone Cloned
ORF length (unspliced) 2676
ORF length (spliced)
Entry clone length 2676
No. of intron 0
Sequence status Finished
Sequence results 1653T:C / 1746T:C / 2258A:G / 2654T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22F8.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAAGACAATTCATTTG
Rev primer name SPAC22F8.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCACATAATGAGTTTAAA
Amino acid length 891
Molecular weight 100.6
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSLSMKKALTL
Localization (YFP) cytosol
Comments for localization bright large dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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