| Gene name |
SPAC4G8.09 |
| Gene ID |
38/F10 |
| Gene synonyms/obsolete |
|
| Gene product |
leucine-tRNA ligase;
involved in leucyl-tRNA aminoacylation; leucine-tRNA ligase
activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2625 |
| ORF length (spliced) |
|
| Entry clone length |
2625 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1158A:G /
1795A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4G8.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGAAATCCGTCGGTAC |
| Rev primer name |
SPAC4G8.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTCGGTGACTAAACTGATG |
| Amino acid length |
874 |
| Molecular weight |
100.8 |
| Isoelectric point (calc.) |
9.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
mitochondrion? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |