| Gene name |
SPAC9.05 |
| Gene ID |
38/F02 |
| Gene synonyms/obsolete |
|
| Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; similar to Sp
SPAC20H4.04 (paralog) |
| Entry clone |
Cloned in 2006
trial |
| ORF length (unspliced) |
2606 |
| ORF length (spliced) |
2520 |
| Entry clone length |
2606 |
| No. of intron |
2 |
| Sequence status |
Partially
sequenced |
| Sequence results |
100% match in both
ends |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC9.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCGATGATTCTTTTAG |
| Rev primer name |
SPAC9.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATCAGCATTCCTTTCATA |
| Amino acid length |
839 |
| Molecular weight |
97.2 |
| Isoelectric point (calc.) |
9.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LATFAYPLNL |
| Localization (YFP) |
nucleolus>nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |