Gene name |
SPCC74.02c |
Gene ID |
38/D08 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical
serine-rich protein; sequence orphan |
Entry clone |
Cloned# |
ORF length (unspliced) |
2462 |
ORF length (spliced) |
2133 |
Entry clone length |
2462 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC74.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAATTGGAATTCCGT |
Rev primer name |
SPCC74.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATAGCATTCGAGTAATTT |
Amino acid length |
710 |
Molecular weight |
77.2 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
500 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LIHLILLVL |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |