Gene name |
SPBC354.05c |
Gene ID |
38/D04 |
Gene synonyms/obsolete |
|
Gene product |
DNA-binding protein;
involved in transcriptional regulation; myc family; basic
helix-loop-helix (bHLH); membrane-tethered transcription
factor; no apparent Scortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
2382 |
ORF length (spliced) |
|
Entry clone length |
2382 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
111A:G / 1030T:C /
1341T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC354.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGTCCTTCCCAACACTC |
Rev primer name |
SPBC354.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGTTGCAACACCAGATTCT |
Amino acid length |
793 |
Molecular weight |
86.8 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
2 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHSILRFLLLL/LRGKLLSELNL |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |