Gene name |
SPAC32A11.01 |
Gene ID |
38/C02 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
sequence orphan |
Entry clone |
Cloned |
ORF length (unspliced) |
2306 |
ORF length (spliced) |
2163 |
Entry clone length |
2306 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
323A:G / 462T:C /
1385G:A / 1651T:C / 1758A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC32A11.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAGAAGCATCTGTAC |
Rev primer name |
SPAC32A11.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAATTTATTTTGAATTTTA |
Amino acid length |
720 |
Molecular weight |
82.4 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFTELASRLEL |
Localization (YFP) |
periphery at site of
septum formation; cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |