| Gene name |
SPAC8C9.15c |
| Gene ID |
37/H05 |
| Gene synonyms/obsolete |
tif225 |
| Gene product |
translation initiation
factor (eif-2b subiunit); nucleotidyltransferase; guanine
nucleotide exchange factor |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2193 |
| ORF length (spliced) |
2037 |
| Entry clone length |
2193 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
2076T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC8C9.15.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCTCCATCCAAAGGGCT |
| Rev primer name |
SPAC8C9.15.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTGACCCTTCTTCACTC |
| Amino acid length |
678 |
| Molecular weight |
76.3 |
| Isoelectric point (calc.) |
4.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLGYFYQLEI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>>cytosol |
| Microscope used for
observation |
Leica |