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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC14G10.05
Gene ID 37/F09
Gene synonyms/obsolete SPCC1020.13c
Gene product phospholipase; DDHD domain; similar to Sp SPAC2G8.02 (paralog)
Entry clone Cloned
ORF length (unspliced) 2158
ORF length (spliced) 2010
Entry clone length 2158
No. of intron 3
Sequence status Finished
Sequence results 2009A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC14G10.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAGAATCATCCCAGGA
Rev primer name SPCC14G10.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAGAGCTGTCCCCTTTGAAT
Amino acid length 669
Molecular weight 77.4
Isoelectric point (calc.) 5.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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