Gene name |
SPCC14G10.05 |
Gene ID |
37/F09 |
Gene synonyms/obsolete |
SPCC1020.13c |
Gene product |
phospholipase; DDHD
domain; similar to Sp SPAC2G8.02 (paralog) |
Entry clone |
Cloned |
ORF length (unspliced) |
2158 |
ORF length (spliced) |
2010 |
Entry clone length |
2158 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
2009A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC14G10.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAAGAATCATCCCAGGA |
Rev primer name |
SPCC14G10.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGAGCTGTCCCCTTTGAAT |
Amino acid length |
669 |
Molecular weight |
77.4 |
Isoelectric point (calc.) |
5.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |