Gene name |
SPCC663.14c |
Gene ID |
37/F04 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
similar to Sp SPAC1F7.03 |
Entry clone |
Cloned |
ORF length (unspliced) |
2153 |
ORF length (spliced) |
2064 |
Entry clone length |
2153 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
317G:A / 1243T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC663.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAGCTAATACTCTTAGC |
Rev primer name |
SPCC663.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAACAGTACGCTGCATTTCA |
Amino acid length |
687 |
Molecular weight |
77.2 |
Isoelectric point (calc.) |
7.8 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTTNLFTLTL/LLLALLFGLFL/LTRVVSFALLI |
Localization (YFP) |
periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |