| Gene name |
SPCC970.10c |
| Gene ID |
37/D01 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); E3 ubiquitin ligase; similar to
Sp SPCC1919.15; involved in ubiquitination; involved in
histone H2B monoubiquitination |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2107 |
| ORF length (spliced) |
2043 |
| Entry clone length |
2107 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
312T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC970.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATCAGAATGGTAAACC |
| Rev primer name |
SPCC970.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGATGTATCGGAATAACA |
| Amino acid length |
680 |
| Molecular weight |
78 |
| Isoelectric point (calc.) |
6.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVDLNDLEL |
| Localization (YFP) |
nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |