Gene name |
SPCC553.09c |
Gene ID |
36/H01 |
Gene synonyms/obsolete |
|
Gene product |
DNA polymerase alpha
subunit |
Entry clone |
Cloned in 2004
trial_also cloned#/ 3' FS at 1st |
ORF length (unspliced) |
1847 |
ORF length (spliced) |
1725 |
Entry clone length |
1847 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC553.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATTTCCTATCGATGA |
Rev primer name |
SPCC553.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAGTTTTAAAATTTCTGCC |
Amino acid length |
574 |
Molecular weight |
64.1 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEVLNKELKI/LNTMPDILIL/LGTFAKLTI |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |