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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A10.05c
Gene ID 36/F07
Gene synonyms/obsolete pof1
Gene product F-box protein; involved in sulfur metabolism; involved in protein ubiquitination
Entry clone Cloned
ORF length (unspliced) 1818
ORF length (spliced)
Entry clone length 1818
No. of intron 0
Sequence status Finished
Sequence results 781C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC57A10.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTACAGGTTATGAATC
Rev primer name SPAC57A10.05.Rv
Rev primer SEQ AGAAAGCTGGGTACGATTGAATCGACACATCG
Amino acid length 605
Molecular weight 67.1
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVRLDFLSL
Localization (YFP) nucleus
Comments for localization one nuclear dot by over expression
Effect of LMB on protein localization no change
Microscope used for observation Confocal, DeltaVision

Image information
YFP 3 images) See all images

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