Gene name |
SPAC17H9.04c |
Gene ID |
36/F05 |
Gene synonyms/obsolete |
|
Gene product |
RNA-binding protein;
zinc finger protein; zfranBP; rrm RNA recognition motif |
Entry clone |
Cloned |
ORF length (unspliced) |
1815 |
ORF length (spliced) |
|
Entry clone length |
1815 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
330A:G / 1059T:C /
1152A:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC17H9.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAAGCTACCAAGTCC |
Rev primer name |
SPAC17H9.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACACGTAAGCATTGAAATCC |
Amino acid length |
604 |
Molecular weight |
66.3 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTNDVTQLEL |
Localization (YFP) |
nucleolus |
Comments for localization |
a few cytoplasmic
dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |