| Gene name |
SPAC17H9.04c |
| Gene ID |
36/F05 |
| Gene synonyms/obsolete |
|
| Gene product |
RNA-binding protein;
zinc finger protein; zfranBP; rrm RNA recognition motif |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1815 |
| ORF length (spliced) |
|
| Entry clone length |
1815 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
330A:G / 1059T:C /
1152A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC17H9.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAAGCTACCAAGTCC |
| Rev primer name |
SPAC17H9.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACACGTAAGCATTGAAATCC |
| Amino acid length |
604 |
| Molecular weight |
66.3 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTNDVTQLEL |
| Localization (YFP) |
nucleolus |
| Comments for localization |
a few cytoplasmic
dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |