Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17H9.04c
Gene ID 36/F05
Gene synonyms/obsolete
Gene product RNA-binding protein; zinc finger protein; zfranBP; rrm RNA recognition motif
Entry clone Cloned
ORF length (unspliced) 1815
ORF length (spliced)
Entry clone length 1815
No. of intron 0
Sequence status Finished
Sequence results 330A:G / 1059T:C / 1152A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17H9.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAAAGCTACCAAGTCC
Rev primer name SPAC17H9.04.Rv
Rev primer SEQ AGAAAGCTGGGTACACGTAAGCATTGAAATCC
Amino acid length 604
Molecular weight 66.3
Isoelectric point (calc.) 7.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LTNDVTQLEL
Localization (YFP) nucleolus
Comments for localization a few cytoplasmic dots
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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