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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC23C11.03
Gene ID 36/D05
Gene synonyms/obsolete
Gene product U3 snoRNP component; ribonucleoprotein (RNP) complex; processome component; involved in rRNA processing
Entry clone Cloned
ORF length (unspliced) 1797
ORF length (spliced)
Entry clone length 1797
No. of intron 0
Sequence status Finished
Sequence results 360T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC23C11.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTGAACTTATAAATTC
Rev primer name SPAC23C11.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGCTTTAGCTGATTTGAA
Amino acid length 598
Molecular weight 67.7
Isoelectric point (calc.) 4.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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