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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC18H10.05
Gene ID 36/B06
Gene synonyms/obsolete
Gene product WD repeat protein; similar to Sp SPAC3H5.08C
Entry clone Cloned
ORF length (unspliced) 1761
ORF length (spliced)
Entry clone length 1761
No. of intron 0
Sequence status Finished
Sequence results 28A:G / 52T:C / 993G:A / 1058T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC18H10.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGCGTTTTGGTTGCTGA
Rev primer name SPBC18H10.05.Rv
Rev primer SEQ AGAAAGCTGGGTAGGTTCGTAATAAACGCTTC
Amino acid length 586
Molecular weight 66
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYNTVTHALVL
Localization (YFP) cytosol=nucleus; periphery at cell tip and site of septum formation
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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