Gene name |
SPBC18H10.05 |
Gene ID |
36/B06 |
Gene synonyms/obsolete |
|
Gene product |
WD repeat protein;
similar to Sp SPAC3H5.08C |
Entry clone |
Cloned |
ORF length (unspliced) |
1761 |
ORF length (spliced) |
|
Entry clone length |
1761 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
28A:G / 52T:C / 993G:A
/ 1058T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC18H10.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGCGTTTTGGTTGCTGA |
Rev primer name |
SPBC18H10.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGTTCGTAATAAACGCTTC |
Amino acid length |
586 |
Molecular weight |
66 |
Isoelectric point (calc.) |
8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYNTVTHALVL |
Localization (YFP) |
cytosol=nucleus;
periphery at cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |