Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11D3.17
Gene ID 36/B03
Gene synonyms/obsolete
Gene product involved in transcriptional regulation; zinc finger protein; zf-C2H2 type; no apparent orthologs
Entry clone Cloned
ORF length (unspliced) 1758
ORF length (spliced)
Entry clone length 1758
No. of intron 0
Sequence status Finished
Sequence results 806C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC11D3.17.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAGATCATGTTTCTGA
Rev primer name SPAC11D3.17.Rv
Rev primer SEQ AGAAAGCTGGGTATGTTGGACGGAACGCTTTG
Amino acid length 585
Molecular weight 67.1
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LRPYFTILPL/LYILEWFLAL/LSILVNGPLLL
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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