| Gene name |
SPBC4F6.03c |
| Gene ID |
36/B01 |
| Gene synonyms/obsolete |
leu3;
SPBC3E7.16c |
| Gene product |
2-isopropylmalate
synthase |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
1755 |
| ORF length (spliced) |
|
| Entry clone length |
1755 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC4F6.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATCTACTTTTGAGGC |
| Rev primer name |
SPBC4F6.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTAGCAAATGCGCGGGAG |
| Amino acid length |
584 |
| Molecular weight |
64 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |