| Gene name |
SPAC29A4.20 |
| Gene ID |
35/G01 |
| Gene synonyms/obsolete |
|
| Gene product |
RNA polymerase II
holoenzyme component; histone acetyltransferase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1730 |
| ORF length (spliced) |
1635 |
| Entry clone length |
1730 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
1047T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC29A4.20.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGACTTCCAGTTTGGC |
| Rev primer name |
SPAC29A4.20.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAGCCATTTAGACATGTAA |
| Amino acid length |
544 |
| Molecular weight |
61.8 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVDLIARILAL |
| Localization (YFP) |
cytosol; cytoplasmic
dots |
| Comments for localization |
bright large dots by
over expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |