Gene name |
SPAC637.07 |
Gene ID |
35/D10 |
Gene synonyms/obsolete |
moe1 |
Gene product |
eIF3 translation
initiation factor 3; involved in microtubule cytoskeleton
organization and biogenesis; involved in microtubule
disassembly, chromosome segregation, and spindle formation;
required for stable association of eIF3 subunits; no apparent
Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1704 |
ORF length (spliced) |
|
Entry clone length |
1704 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC637.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAACTGGATTTAAATT |
Rev primer name |
SPAC637.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCAGTAGAAGAAGCTTCA |
Amino acid length |
567 |
Molecular weight |
62.6 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |