Gene name |
SPBC16G5.09 |
Gene ID |
34/F04 |
Gene synonyms/obsolete |
|
Gene product |
serine
carboxypeptidase family; peptidase family S10; predicted
N-terminal signal sequence |
Entry clone |
Cloned |
ORF length (unspliced) |
1647 |
ORF length (spliced) |
1533 |
Entry clone length |
1647 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC16G5.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTCGAAGTTACTGAA |
Rev primer name |
SPBC16G5.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGAGTTGGAGATATAGGT |
Amino acid length |
510 |
Molecular weight |
57.6 |
Isoelectric point (calc.) |
4.4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVNLGGLAI/LGLIPVALTI |
Localization (YFP) |
periphery;
vacuole |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |