| Gene name |
SPBC16G5.09 |
| Gene ID |
34/F04 |
| Gene synonyms/obsolete |
|
| Gene product |
serine
carboxypeptidase family; peptidase family S10; predicted
N-terminal signal sequence |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1647 |
| ORF length (spliced) |
1533 |
| Entry clone length |
1647 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC16G5.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTTCGAAGTTACTGAA |
| Rev primer name |
SPBC16G5.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGAGTTGGAGATATAGGT |
| Amino acid length |
510 |
| Molecular weight |
57.6 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVNLGGLAI/LGLIPVALTI |
| Localization (YFP) |
periphery;
vacuole |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |