Gene name |
SPCC548.06c |
Gene ID |
34/E10 |
Gene synonyms/obsolete |
|
Gene product |
hexose transporter;
similar to Sp GHT1 and GHT2 and GHT4 and GHT5 and GHT6 and
GHT3 and SPBC1348.14C; tandem duplication |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1644 |
ORF length (spliced) |
|
Entry clone length |
1644 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC548.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGTAAGACATTGACCAT |
Rev primer name |
SPCC548.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCGTAGCGATCTTGTTCA |
Amino acid length |
547 |
Molecular weight |
60.1 |
Isoelectric point (calc.) |
5.9 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LEEINELYL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |